human colon tissue were subjected to SDS PAGE followed by western blot with FNab06896(PSME3 antibody) at dilution of 1:500
IP Result of anti-PSME3 (IP:FNab06896, 3ug; Detection:FNab06896 1:500) with COLO 320 cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human colon cancer using FNab06896(PSME3 antibody) at dilution of 1:50
Background
Subunit of the 11S REG-gamma(also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring(PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination-and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition(PubMed:25361978).