Products
PKM antibody
- Product Name
- PKM antibody
- Catalogue No.
- FNab06492
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- PKM2 pyruvate kinase, muscle
- Alternative Names
- OIP3 antibody, PK2 antibody, PK3 antibody, PKM2 antibody
- UniProt ID
- P14618
- Observed MW
- 58 kDa
Application
- Tested Applications
- ELISA, IHC, WB
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab06492(PKM antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer using FNab06492(PKM antibody) at dilution of 1:50
- Background
- Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate(PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
