Products
PCNA antibody
- Product Name
- PCNA antibody
- Catalogue No.
- FNab06217
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 8F9
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- proliferating cell nuclear antigen
- Alternative Names
- Proliferating cell nuclear antigen antibody, Cyclin antibody
- UniProt ID
- P12004
- Observed MW
- 38 kDa
Application
- Tested Applications
- ELISA, WB, IHC, FC, IP
- Recommended dilution
- WB: 1:5000-1:50000; IP: 1:200-1:1000; IHC: 1:500-1:2000; IF: 1:50-1:500
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab06217(PCNA Antibody) at dilution of 1:10000
IP Result of anti-PCNA (IP:FNab06217, 4ug; Detection:FNab06217 1:300) with HepG2 cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab06217(PCNA antibody) at dilution of 1:800
Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using FNab06217 (PCNA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)
- Background
- Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic(AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response(DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways(PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion(TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
