Products
PARD3 antibody
- Product Name
- PARD3 antibody
- Catalogue No.
- FNab06146
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Partitioning defective 3 homolog
- Alternative Names
- ASIP antibody, Baz antibody, Bazooka antibody, CTCL tumor antigen se2 5 antibody, FLJ21015 antibody, PAR 3 antibody, PAR3 antibody, PAR3 alpha antibody, PAR3A antibody, PAR3alpha antibody, PARD 3 antibody, PARD3 antibody, PARD3A antibody, SE2 5L16 antibody, SE2 5LT1 antibody, SE2 5T2 antibody
- UniProt ID
- Q8TEW0
- Observed MW
- 100 kDa, 150 kDa, and 180 kDa
Application
- Tested Applications
- ELISA, WB, IHC, FC, IP
- Recommended dilution
- WB: 1:500-1:1000; IP: 1:200-1:1000; IHC: 1:50-1:200; IF: 1:50-1:500
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab06146(PARD3 antibody) at dilution of 1:500
IP Result of anti-PARD3 (IP:FNab06146, 4ug; Detection:FNab06146 1:200) with MCF-7 cells lysate 2900ug.
Immunohistochemistry of paraffin-embedded human colon cancer using FNab06146(PARD3 antibody) at dilution of 1:50
Immunofluorescent analysis of fixed MCF-7 cells using FNab06146 (PARD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Involved in Schwann cell peripheral myelination(By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.
