Products
NENF antibody
- Product Name
- NENF antibody
- Catalogue No.
- FNab05661
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2a
- Clone ID
- 7C8
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- neuron derived neurotrophic factor
- Alternative Names
- CIR2 antibody, NENF antibody, NEUDESIN antibody, SCIRP10 antibody, SPUF antibody, SPUF protein antibody
- UniProt ID
- Q9UMX5
- Observed MW
- 18 kDa
Application
- Tested Applications
- ELISA, IHC, WB,IF
- Recommended dilution
- WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:500
Validated Images
human brain tissue were subjected to SDS PAGE followed by western blot with FNab05661(NENF antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney slide using FNab05661(NENF antibody) at dilution of 1:50
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using FNab05661 ( NENF Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
- Background
- Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance(By similarity). Plays a role in the human tumorigenesis(PubMed:22748190).
