Products
MIA3 antibody
- Product Name
- MIA3 antibody
- Catalogue No.
- FNab05174
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- melanoma inhibitory activity family, member 3
- Alternative Names
- KIAA0268 antibody, TANGO antibody, TANGO1 antibody
- UniProt ID
- Q5JRA6
- Observed MW
- 250 kDa
Application
- Tested Applications
- ELISA, IP, WB
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000
Validated Images
HEK-293T cells were subjected to SDS PAGE followed by western blot with FNab05174(MIA3 antibody) at dilution of 1:500
IP Result of anti-MIA3 (IP:FNab05174, 5ug; Detection:FNab05174 1:1000) with HeLa cells lysate 3000ug.
- Background
- Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII(COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum(PubMed:27138255).
