Products
MED15 antibody
- Product Name
- MED15 antibody
- Catalogue No.
- FNab05089
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- mediator complex subunit 15
- Alternative Names
- ARC105 antibody, CTG7A antibody, PCQAP antibody, TIG1 antibody, TNRC7 antibody
- UniProt ID
- Q96RN5
- Observed MW
- 105 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
K-562 cells were subjected to SDS PAGE followed by western blot with FNab05089(MED15 antibody) at dilution of 1:500
IP Result of anti-MED15 (IP:FNab05089, 3ug; Detection:FNab05089 1:500) with K-562 cells lysate 3440ug.
Immunohistochemistry of paraffin-embedded human prostate cancer using FNab05089(MED15 antibody) at dilution of 1:100
- Background
- Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.
