mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with FNab04974(MAP1B antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using FNab04974(MAP1B Antibody) at dilution of 1:50
Immunofluorescent analysis of SH-SY5Y cells using FNab04974 ( MAP1B Antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Background
Facilitates tyrosination of alpha-tubulin in neuronal microtubules(By similarity). Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.