HepG2 cells were subjected to SDS PAGE followed by western blot with FNab04623(KLHL3 antibody) at dilution of 1:800
IP Result of anti-KLHL3 (IP:FNab04623, 4ug; Detection:FNab04623 1:700) with HepG2 cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human liver using FNab04623(KLHL3 antibody) at dilution of 1:100
Background
Substrate-specific adapter of a BCR(BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex acts by mediating ubiquitination of WNK4, an inhibitor of potassium channel KCNJ1, leading to WNK4 degradation.