Products
JNK antibody
- Product Name
- JNK antibody
- Catalogue No.
- FNab04444
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- mitogen-activated protein kinase 8
- Alternative Names
- c Jun N terminal kinase 1 antibody, JNK antibody, JNK 46 antibody, JNK1 antibody, JNK1A2 antibody, JNK21B1/2 antibody, MAP kinase 8 antibody, MAPK 8 antibody, MAPK8 antibody, PRKM8 antibody, SAPK1 antibody
- UniProt ID
- P45983
- Observed MW
- 46 kDa, 55 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab04444(JNK antibody) at dilution of 1:1000
IP Result of anti-JNK (IP: FNab04444, 3ug; Detection: FNab04444 1:1000) with HeLa cells lysate 1000ug.
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using FNab04444(JNK Antibody) at dilution of 1:50
- Background
- MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has 4 isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa.
