Products
HLA class I (HLA-A) antibody
- Product Name
- HLA class I (HLA-A) antibody
- Catalogue No.
- FNab03896
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2a
- Clone ID
- 0D3
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- major histocompatibility complex, class I, A
- Alternative Names
- HLA A antibody, HLA class I antibody, HLA class I (HLA-A) antibody, HLA class I ABC antibody, HLA-A antibody, MHC class I antigen A*3 antibody
- UniProt ID
- P04439/P30443
- Observed MW
- 44 kDa
Application
- Tested Applications
- ELISA, WB, IHC, FC
- Recommended dilution
- WB: 1:1000-1:8000; IHC: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab03896(HLA class I(HLA-A) Antibody) at dilution of 1:4000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using FNab03896(HLA class I(HLA-A) Antibody) at dilution of 1:200
- Background
- HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.
