Products
GRP75 antibody
- Product Name
- GRP75 antibody
- Catalogue No.
- FNab03661
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- heat shock 70kDa protein 9(mortalin)
- Alternative Names
- CSA antibody, GRP 75 antibody, GRP75 antibody, Heat shock 70 kDa protein 9 antibody, HSPA9 antibody, HSPA9B antibody, Mortalin antibody, MOT antibody, mot 2 antibody, MOT2 antibody, MTHSP75 antibody, PBP74 antibody, Peptide binding protein 74 antibody
- UniProt ID
- P38646
- Observed MW
- 74 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, FC, IP
- Recommended dilution
- WB: 1:1000-1:4000; IP: 1:1000-1:4000; IHC: 1:20-1:200; IF: 1:10-1:100
Validated Images
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with FNab03661(GRP75 antibody) at dilution of 1:2000
IP Result of anti-GRP75 (IP:FNab03661, 4ug; Detection:FNab03661 1:2000) with mouse brain tissue lysate 3680ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab03661(GRP75 antibody) at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using FNab03661 (GRP75 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
- Background
- GRP75(also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA(HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson’s disease.
