Products
Cytokeratin 1-specific antibody
- Product Name
- Cytokeratin 1-specific antibody
- Catalogue No.
- FNab02211
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- keratin 1
- Alternative Names
- 67 kDa cytokeratin antibody, CK 1 antibody, CK1 antibody, Cytokeratin 1 antibody, keratin 1 antibody, KRT1 antibody
- UniProt ID
- P04264
- Observed MW
- 67 kDa
Application
- Tested Applications
- ELISA, IP, IF, WB, IHC
- Recommended dilution
- WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
mouse skin tissue were subjected to SDS PAGE followed by western blot with FNab02211(KRT1-specific Antibody) at dilution of 1:1000
IP Result of anti-KRT1-specific (IP: FNab02211, 3ug; Detection: FNab02211 1:500) with A431 cells lysate 3500ug.
Immunohistochemistry of paraffin-embedded human lung cancer using FNab02211(KRT1-specific antibody) at dilution of 1:50
Immunofluorescent analysis of A431 cells using FNab02211 ( KRT1-specific Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
- Background
- Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. This antibody is specifically against ketatin I.
