Products
Cytokeratin 18 antibody
- Product Name
- Cytokeratin 18 antibody
- Catalogue No.
- FNab02206
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2a
- Clone ID
- 6E3
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- keratin 18
- Alternative Names
- CK 18 antibody, CYK18 antibody, Cytokeratin 18 antibody, K18 antibody, keratin 18 antibody, KRT18 antibody
- UniProt ID
- P05783
- Observed MW
- 48 kDa
Application
- Tested Applications
- ELISA, WB, FC, IF, IHC
- Recommended dilution
- WB: 1:5000-1:30000; IHC: 1:500-1:1000; IF: 1:100-1:300
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab02206(KRT18 Antibody) at dilution of 1:20000
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using FNab02206(KRT18 Antibody) at dilution of 1:200
Immunofluorescent analysis of HepG2 cells using FNab02206 ( KRT18 Antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
- Background
- Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.
