Products

CLSPN antibody

Catalogue No.:FNab01775Reativity:Human
Host:RabbitTested Application:ELISA, WB, IF, IP
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
CLSPN antibody
Catalogue No.
FNab01775
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
claspin homolog(Xenopus laevis)
UniProt ID
Q9HAW4
Observed MW
240 kDa
Application
Tested Applications
ELISA, WB, IF, IP
Recommended dilution
WB: 1:200-1:1000; IP: 1:500-1:2000; IF: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab01775( Claspin antibody) at dilution of 1:300
IP Result of anti-Claspin (IP:FNab01775, 5ug; Detection:FNab01775 1:1000) with HEK-293 cells lysate 2400ug.
Background
Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex(pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.