HeLa cells were subjected to SDS PAGE followed by western blot withFNab01523(CDC25C antibody) at dilution of 1:4000
IP Result of anti-CDC25C (IP:FNab01523, 4ug; Detection:FNab01523 1:1000) with K-562 cells lysate 3600ug.
Background
Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.