CD71 antibody

Synonyms:CD71 antibody, p90 antibody, sTfR antibody, T9 antibody, TFR antibody, TFR1 antibody, TFRC antibody, TR antibody, Transferrin receptor protein 1 antibody, TRFR antibody
Catalogue No.:FNab01497Reactivity:Human
Host:MouseTested Application:ELISA, IHC, WB, IF, FC, IP
Clonality:monoclonalIsotype:IgG2a
  • SPECIFICATIONS
Product Name
CD71 antibody
Catalogue No.
FNab01497
Size
100μg
Form
liquid
Purification
Protein A+G purification
Purity
≥95% as determined by SDS-PAGE
Clonality
monoclonal
Isotype
IgG2a
Clone ID
4F3
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
transferrin receptor protein 1
Alternative Names
CD71 antibody, p90 antibody, sTfR antibody, T9 antibody, TFR antibody, TFR1 antibody, TFRC antibody, TR antibody, Transferrin receptor protein 1 antibody, TRFR antibody
UniProt ID
P02786
Observed MW
90 kDa
Application
Tested Applications
ELISA, IHC, WB, IF, FC, IP
Recommended dilution
WB: 1:1000-1:4000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab01497(CD71 Antibody) at dilution of 1:2000
IP result of anti-CD71(IP:FNab01497, 4ug; Detection:FNab01497 1:500) with HeLa cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab01497(CD71 Antibody) at dilution of 1:200
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using FNab01497 ( CD71 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Background
CD71, also known as transferrin receptor protein 1(TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.