Products
BHLHE22 antibody
- Product Name
- BHLHE22 antibody
- Catalogue No.
- FNab00887
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- basic helix-loop-helix family, member e22
- Alternative Names
- BHLHB5 antibody, TNRC20 antibody
- UniProt ID
- Q8NFJ8
- Observed MW
- 37 kDa
Application
- Tested Applications
- ELISA, WB, IF
- Recommended dilution
- WB: 1:200-1:1000; IF: 1:10-1:100
Validated Images
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with FNab00887(BHLHE22 Antibody) at dilution of 1:300
Immunofluorescent analysis of SH-SY5Y cells using FNab00887 (BHLHE22 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Inhibits DNA binding of TCF3/E47 homodimers and TCF3(E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA(By similarity). In the brain, may function as an area-specific transcription factor that regulates the postmitotic acquisition of area identities and elucidate the genetic hierarchy between progenitors and postmitotic neurons driving neocortical arealization. May be required for the survival of a specific population of inhibitory neurons in the superficial laminae of the spinal chord dorsal horn that may regulate pruritis. Seems to play a crucial role in the retinogenesis, in the specification of amacrine and bipolar subtypes. Forms with PRDM8 a transcriptional repressor complex controlling genes involved in neural development and neuronal differentiation.
