Products
BCL2L1 antibody
- Product Name
- BCL2L1 antibody
- Catalogue No.
- FNab00843
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2a
- Clone ID
- 2A9
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- BCL2-like 1
- Alternative Names
- BCL2L antibody, BCLX antibody
- UniProt ID
- Q07817
- Observed MW
- 35 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:2000-1:20000; IHC: 1:200-1:600; IF: 1:10-1:100
Validated Images
Raw 264.7 cells were subjected to SDS PAGE followed by western blot with FNab00843(Bcl-xL Antibody) at dilution of 1:20000
Immunohistochemistry of paraffin-embedded human testis using FNab00843(Bcl-xL antibody) at dilution of 1:50
Immunofluorescent analysis of Hela cells, using BCL2L1 antibody FNab00843 at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
- Background
- Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel(VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release(PubMed:17418785). Isoform Bcl-X(S) promotes apoptosis.
