Products
ATG4C antibody
- Product Name
- ATG4C antibody
- Catalogue No.
- FNab00675
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- ATG4 autophagy related 4 homolog C
- Alternative Names
- APG4C antibody, AUTL1 antibody, AUTL3 antibody
- UniProt ID
- Q96DT6
- Observed MW
- 52-55 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
mouse testis tissue were subjected to SDS PAGE followed by western blot with FNab00675(ATG4C antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human heart using FNab00675(ATG4C antibody) at dilution of 1:50
- Background
- Cysteine protease required for the cytoplasm to vacuole transport(Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation(By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine(PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
