Products
ATF4 antibody
- Product Name
- ATF4 antibody
- Catalogue No.
- FNab00662
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- activating transcription factor 4(tax-responsive enhancer element B67)
- Alternative Names
- ATF4 antibody, CREB 2 antibody, CREB2 antibody, DNA binding protein TAXREB67 antibody, TAXREB67 antibody, TXREB antibody
- UniProt ID
- P18848
- Observed MW
- 50-58 kDa, 35-38 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP, FC
- Recommended dilution
- WB: 1:500-1:1000; IP: 1:200-1:1000; IHC: 1:50-1:500; IF: 1:50-1:500
Validated Images
A431 cells were subjected to SDS PAGE followed by western blot with FNab00662(ATF4 antibody) at dilution of 1:500
IP Result of anti-ATF4 (IP:FNab00662, 3ug; Detection:FNab00662 1:400) with HEK-293 cells lysate 6000ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab00662(ATF4 antibody) at dilution of 1:100
- Background
- Transcriptional activator. Binds the cAMP response element(CRE)(consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production(By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase(ASNS) in response to endoplasmic reticulum(ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements(CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors(PubMed:26086088).
