Products
Amylase, alpha antibody
- Product Name
- Amylase, alpha antibody
- Catalogue No.
- FNab00383
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG2b
- Clone ID
- 1C7
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- amylase, alpha 2A(pancreatic)
- Alternative Names
- AMY2A antibody, Amylase antibody, alpha antibody, Pancreatic alpha amylase antibody
- UniProt ID
- P04746
- Observed MW
- 58 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:1000-1:4000; IHC: 1:20-1:200; IF: 1:10-1:100
Validated Images
human saliva were subjected to SDS PAGE followed by western blot with FNab00383(AMY2A antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using FNab00383(AMY2A Antibody) at dilution of 1:50
Immunofluorescent analysis of BxPC-3 cells using FNab00383 (Amylase, alpha antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)
- Background
- The complete human amylase gene contains five intact genes: two pancreatic genes(AMY2A and AMY2B) and three salivary genes(AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258).AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956). This antibody can recognize both AMY1 and AMY2.
