Products
AMFR antibody
- Product Name
- AMFR antibody
- Catalogue No.
- FNab00358
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- autocrine motility factor receptor
- Alternative Names
- AMF receptor antibody, isoform 2 antibody, AMFR antibody, GP78 antibody, RING finger protein 45 antibody, RNF45 antibody
- UniProt ID
- Q9UKV5
- Observed MW
- 73 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:5000; IF: 1:10-1:100; IHC: 1:20-1:200; IP: 1:200-1:2000
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab00358(AMFR antibody) at dilution of 1:800
IP result of anti-AMFR(IP:FNab00358, 4ug; Detection:FNab00358 1:300) with MCF-7 cells lysate 2400 ug
Immunohistochemistry of paraffin-embedded human ovary using FNab00358(AMFR antibody) at dilution of 1:100
- Background
- E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation(ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.
