Products

Rat 5-LO(Arachidonate 5-Lipoxygenase) ELISA Kit

ER0199 Catalogue No.
5-LO ELISA Kit, Alox5 ELISA Kit, 5-LO ELISA Kit, 5LPG ELISA Kit, LOG5 ELISA Kit, LOX5A ELISA Kit, 5-lipoxygenase ELISA Kit, 5-LOX ELISA Kit, arachidonic 5-lipoxygenase alpha-10 isoform ELISA Kit, arachidonic 5-lipoxygenase delta-10-13 isoform ELISA Kit, arachidonic 5-lipoxygenase delta-13 isoform ELISA Kit, arachidonic 5-lipoxygenase delta-p10 isoform ELISA Kit, arachidonic acid 5-lipoxygenase ELISA Kit, leukotriene A4 synthase ELISA Kit, LOG5 ELISA Kit
Reactivity
Rat
Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Product Name
Rat 5-LO(Arachidonate 5-Lipoxygenase) ELISA Kit
Catalogue No.
ER0199
Alias
5-LO ELISA Kit, Alox5 ELISA Kit, 5-LO ELISA Kit, 5LPG ELISA Kit, LOG5 ELISA Kit, LOX5A ELISA Kit, 5-lipoxygenase ELISA Kit, 5-LOX ELISA Kit, arachidonic 5-lipoxygenase alpha-10 isoform ELISA Kit, arachidonic 5-lipoxygenase delta-10-13 isoform ELISA Kit, arachidonic 5-lipoxygenase delta-13 isoform ELISA Kit, arachidonic 5-lipoxygenase delta-p10 isoform ELISA Kit, arachidonic acid 5-lipoxygenase ELISA Kit, leukotriene A4 synthase ELISA Kit, LOG5 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
5-LO ELISA Kit allows for the in vitro quantitative determination of 5-LO concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
P12527
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of 5-LO and the recovery rates were calculated by comparing the measured value to the expected amount of 5-LO in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)92-10299
EDTA plasma(n=5)89-10397
heparin plasma(n=5)85-10091
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-LO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)86-105%85-104%85-103%
EDTA plasma(n=5)86-98%89-99%83-99%
heparin plasma(n=5)83-100%85-97%81-93%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only