Products

Mouse Gapdh(Glyceraldehyde-3-phosphate dehydrogenase) ELISA Kit

EM0700 Catalogue No.
Gapdh|Peptidyl-cysteine S-nitrosylase GAPDH|GAPD|G3PD|G3PDH|aging-associated gene 9 protein ELISA Kit
Reactivity
Mouse
Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Product Name
Mouse Gapdh(Glyceraldehyde-3-phosphate dehydrogenase) ELISA Kit
Catalogue No.
EM0700
Alias
Gapdh|Peptidyl-cysteine S-nitrosylase GAPDH|GAPD|G3PD|G3PDH|aging-associated gene 9 protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Gapdh ELISA Kit allows for the in vitro quantitative determination of Gapdh concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Mouse
UniProt ID
P16858
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of Gapdh and the recovery rates were calculated by comparing the measured value to the expected amount of Gapdh in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)87-9893
EDTA plasma(n=5)91-10195
heparin plasma(n=5)85-9791
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gapdh and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)90-105%85-101%85-102%85-104%
EDTA plasma(n=5)93-98%90-98%84-101%83-98%
heparin plasma(n=5)82-96%81-97%80-98%85-96%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only