Products

Human DHFR(Dihydrofolate reductase) ELISA Kit

EH2463 Catalogue No.
DHFR ELISA Kit, DHFR ELISA Kit, DHFRP1 ELISA Kit, DYR ELISA Kit, DHFRP1 ELISA Kit, dihydrofolate reductase ELISA Kit, DYR ELISA Kit, EC 1.5.1.3 ELISA Kit
Reactivity
Human
Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Product Name
Human DHFR(Dihydrofolate reductase) ELISA Kit
Catalogue No.
EH2463
Alias
DHFR ELISA Kit, DHFR ELISA Kit, DHFRP1 ELISA Kit, DYR ELISA Kit, DHFRP1 ELISA Kit, dihydrofolate reductase ELISA Kit, DYR ELISA Kit, EC 1.5.1.3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
DHFR ELISA Kit allows for the in vitro quantitative determination of DHFR concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
P00374
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of DHFR and the recovery rates were calculated by comparing the measured value to the expected amount of DHFR in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-10293
EDTA plasma(n=5)89-9894
heparin plasma(n=5)91-10298
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DHFR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)87-98%92-105%95-104%
EDTA plasma(n=5)89-98%82-100%82-91%
heparin plasma(n=5)80-98%83-93%85-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only