Various lysates were subjected to SDS PAGE followed by western blot with FNa10371( SIRT1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon tissue slide using FNab10371( SIRT1 Antibody) at dilution of 1:100
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using FNab10371 (SIRT1 antibody) at dilution of 1:50 and FITC-conjugated Goat Anti-Rabbit IgG(H+L).
Background
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.