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CD44 antibody

Antibody
- Primary Antibody
  - Unconjugated Primary Antibodies

Research Area:

Synonyms：	CD44 antigen\|CDw44\|Epican\|Extracellular matrix receptor III (ECMR-III)\|GP90 lymphocyte homing/adhesion receptor\|HUTCH-I\|Heparan sulfate proteoglycan\|Hermes antigen\|Hyaluronate receptor\|Phagocytic glycoprotein 1 (PGP-1)\|Phagocytic glycoprotein I (PGP-I)\|CD44\|LHR\|MDU2\|MDU3\|MIC4 antibody
Catalogue No.：	FNab01483	Reactivity：	Human
Host：	Mouse	Tested Application：	ELISA, WB, IHC, IF, FC
Clonality：	monoclonal	Isotype：	IgG2a

Manual MSDS

Size	Price
100µg	Inquiry

Dispatch Time: About 3 working days

SPECIFICATIONS
CITATIONS
FIGURES
CONDITIONS
FAQS

Product Name: CD44 antibody
Catalogue No.: FNab01483
Size: 100μg
Form: liquid
Purification: Protein A+G purification
Purity: ≥95% as determined by SDS-PAGE
Clonality: monoclonal
Isotype: IgG2a
Clone ID: 7D6
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Immunogen

Immunogen: CD44 molecule(Indian blood group)
Alternative Names: CD44 antigen|CDw44|Epican|Extracellular matrix receptor III (ECMR-III)|GP90 lymphocyte homing/adhesion receptor|HUTCH-I|Heparan sulfate proteoglycan|Hermes antigen|Hyaluronate receptor|Phagocytic glycoprotein 1 (PGP-1)|Phagocytic glycoprotein I (PGP-I)|CD44|LHR|MDU2|MDU3|MIC4 antibody
UniProt ID: P16070
Observed MW: 80-95 kDa

Application

Tested Applications: ELISA, WB, IHC, IF, FC
Recommended dilution: WB: 1:500-1:5000; IHC: 1:50-1:200; IF: 1:10-1:100

Validated Images

HeLa cells were subjected to SDS PAGE followed by western blot with FNab01483(CD44 antibody) at dilution of 1:1000

Immunohistochemistry of paraffin-embedded human lung cancer slide using FNab01483(CD44 Antibody) at dilution of 1:50

Background: Receptor for hyaluronic acid(HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases(MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T(iTreg) cell stability and suppressive function(By similarity).

Antiproliferative effect of L1CAM-specific aptamers in human glioblastoma cell cultures

Journal:

Neuroscience and Behavioral Physiology

Author:

Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia

Cited Date:

2025-01-10

Product:

CD44 antibody

How many times can antibodies be recycled?

First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.

Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.

What are components of FineTest antibody buffer?

Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.

How about the storage temperature and duration of FineTest antibodies?

Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.

Is dilution required for FineTest antibodies? What’s the dilute solution?

Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).

How to retrieve antibodies for immunohistochemistry?

Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)

Heat induced antibody retrieval:

Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;

Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.

How to choose secondary antibodies?

(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.

(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).

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