Human PDE5A(cGMP-specific 3′,5′-cyclic phosphodiesterase) ELISA Kit

Manual
  • Specifications
Product name

Human PDE5A(cGMP-specific 3′,5′-cyclic phosphodiesterase) ELISA Kit

Catalogue No.

EH0904

Alias

PDE5A(cGMP-specific 3′,5′-cyclic phosphodiesterase ELISA Kit, ) ELISA Kit, cGMP-binding cGMP-specific phosphodiesterase(CGB-PDE) ELISA Kit, PDE5 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PDE5A ELISA Kit allows for the in vitro quantitative determination of PDE5A concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human PDE5A(cGMP-specific 3′,5′-cyclic phosphodiesterase) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PDE5A and the recovery rates were calculated by comparing the measured value to the expected amount of PDE5A in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-102 95
EDTA plasma(n=5) 89-104 96
heparin plasma(n=5) 87-95 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE5A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-100% 85-100% 86-103% 97-104%
EDTA plasma(n=5) 83-97% 82-100% 88-100% 83-96%
heparin plasma(n=5) 81-96% 82-97% 83-99% 81-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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