Human BRCA1(Breast cancer type 1 susceptibility protein) ELISA Kit

Manual
  • Specifications
Product name

Human BRCA1(Breast cancer type 1 susceptibility protein) ELISA Kit

Catalogue No.

EH1088

Alias

BRCA1 ELISA Kit, RNF53 ELISA Kit, Breast cancer type 1 susceptibility protein ELISA Kit, RING finger protein 53 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

BRCA1 ELISA Kit allows for the in vitro quantitative determination of BRCA1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.312-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human BRCA1(Breast cancer type 1 susceptibility protein) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of BRCA1 and the recovery rates were calculated by comparing the measured value to the expected amount of BRCA1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-98 94
EDTA plasma(n=5) 93-104 99
heparin plasma(n=5) 90-102 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BRCA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-104% 89-101% 86-104% 85-97%
EDTA plasma(n=5) 82-95% 84-93% 84-101% 89-101%
heparin plasma(n=5) 82-97% 87-95% 80-93% 84-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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027-87384275