Recombinant Mouse GM-CSF

Alternative Names:colony stimulating factor 2 (granulocyte-macrophage) Protein, Colony-stimulating factor Protein, CSF Protein, CSF2 Protein, CSF-2 Protein, GMCSF Protein, GM-CSF Protein, GMCSFgranulocyte-macrophage colony-stimulating factor Protein, granulocyte-macrophage colony stimulating factor Protein, MGC131935 Protein, MGC138897 Protein, Molgramostim Protein, molgramostin Protein, Sargramostim Protein
Catalogue No.:P9321Uniprot ID:P01587
Host:HEK293 cellsExpression Region:18-141
Mol. Weight:23 kDaTags:C-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant Mouse GM-CSF
Catalogue No.
P9321
Species
Mouse
Host
HEK293 cells
Expression Region
18-141
Mol. Weight
23 kDa
Tags
C-terminal His Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 20-80 pg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
colony stimulating factor 2 (granulocyte-macrophage) Protein, Colony-stimulating factor Protein, CSF Protein, CSF2 Protein, CSF-2 Protein, GMCSF Protein, GM-CSF Protein, GMCSFgranulocyte-macrophage colony-stimulating factor Protein, granulocyte-macrophage colony stimulating factor Protein, MGC131935 Protein, MGC138897 Protein, Molgramostim Protein, molgramostin Protein, Sargramostim Protein
SDS-PAGE