Recombinant Human MCP3

Alternative Names:C-C motif chemokine 7 Protein, Ccl7 Protein, CCL7_HUMAN Protein, Chemokine CC motif ligand 7 Protein, FIC Protein, MARC Protein, MCP-3 Protein, Monocyte chemoattractant protein 3 Protein, Monocyte chemotactic protein 3 Protein, NC28 Protein, RP23-350G1.4 Protein, SCYA6 Protein, SCYA7 Protein, Small-inducible cytokine A7 Protein
Catalogue No.:P5565Uniprot ID:P80098
Host:E.ColiExpression Region:24-99
Mol. Weight:8.3 kDaTags:N-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant Human MCP3
Catalogue No.
P5565
Species
Human
Host
E.Coli
Expression Region
24-99
Mol. Weight
8.3 kDa
Tags
N-terminal His Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured by its ability to chemoattract 2-day cultured human monocytes. The ED50 for this effect is 0.1-0.5 μg/mL
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
C-C motif chemokine 7 Protein, Ccl7 Protein, CCL7_HUMAN Protein, Chemokine CC motif ligand 7 Protein, FIC Protein, MARC Protein, MCP-3 Protein, Monocyte chemoattractant protein 3 Protein, Monocyte chemotactic protein 3 Protein, NC28 Protein, RP23-350G1.4 Protein, SCYA6 Protein, SCYA7 Protein, Small-inducible cytokine A7 Protein
SDS-PAGE