Recombinant Rat IL-10

Alternative Names:CSIF Protein, Cytokine synthesis inhibitory factor Protein, GVHDS Protein, IL 10 Protein, IL-10 Protein, IL10 Protein, IL10_HUMAN Protein, IL10A Protein, Interleukin 10 Protein, Interleukin-10 Protein, MGC126450 Protein, MGC126451 Protein, T-cell growth inhibitory factor Protein, TGIF Protein
Catalogue No.:P5042Uniprot ID:P29456
Host:E.ColiExpression Region:19-178
Mol. Weight:17.5 kDaTags:N-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant Rat IL-10
Catalogue No.
P5042
Species
Rat
Host
E.Coli
Expression Region
19-178
Mol. Weight
17.5 kDa
Tags
N-terminal His Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is 0.2-0.6 ng/mL
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
CSIF Protein, Cytokine synthesis inhibitory factor Protein, GVHDS Protein, IL 10 Protein, IL-10 Protein, IL10 Protein, IL10_HUMAN Protein, IL10A Protein, Interleukin 10 Protein, Interleukin-10 Protein, MGC126450 Protein, MGC126451 Protein, T-cell growth inhibitory factor Protein, TGIF Protein
SDS-PAGE