Recombinant Rat IL-6

Alternative Names:Interleukin BSF 2 Protein, B cell differentiation factor Protein, B cell stimulatory factor 2 Protein, B-cell stimulatory factor 2 Protein, BSF 2 Protein, BSF-2 Protein, BSF2 Protein, CDF Protein, CTL differentiation factor Protein, Cytotoxic T cell differentiation factor Protein, Hepatocyte stimulating factor Protein, Hepatocyte stimulatory factor Protein, HGF Protein, HSF Protein
Catalogue No.:P4890Uniprot ID:P20607
Host:E.ColiExpression Region:25-211
Mol. Weight:20.5 kDaTags:N-terminal His Tag or N-terminal His-IF2DI Tag, determined during production process
  • SPECIFICATIONS
Product Name
Recombinant Rat IL-6
Catalogue No.
P4890
Species
Rat
Host
E.Coli
Expression Region
25-211
Mol. Weight
20.5 kDa
Tags
N-terminal His Tag or N-terminal His-IF2DI Tag, determined during production process
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. The ED50 for this effect is 0.2-0.5 ng/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
Interleukin BSF 2 Protein, B cell differentiation factor Protein, B cell stimulatory factor 2 Protein, B-cell stimulatory factor 2 Protein, BSF 2 Protein, BSF-2 Protein, BSF2 Protein, CDF Protein, CTL differentiation factor Protein, Cytotoxic T cell differentiation factor Protein, Hepatocyte stimulating factor Protein, Hepatocyte stimulatory factor Protein, HGF Protein, HSF Protein