Products
Recombinant Rat TNF alpha
- Product Name
- Recombinant Rat TNF alpha
- Catalogue No.
- P4812
- Species
- Rat
- Host
- E.Coli
- Expression Region
- 80-235
- Mol. Weight
- 17.1 kDa
- Tags
- N-terminal His Tag
- Purity
- Greater than 95% as determined by SDS-PAGE
- Application
- Western Blot, ELISA
- Activity
- Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin. The ED50 for this effect is 10-30 pg/mL.
- Formulation
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
- Reconstitution
- Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
- Storage
- The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
- Alternative Names
- APC1 Protein, APC1 protein Protein, Cachectin Protein, DIF Protein, Differentiation inducing factor Protein, Macrophage cytotoxic factor Protein, Tnf Protein, TNF superfamily member 2 Protein, TNF superfamily Protein, member 2 Protein, TNF Protein, macrophage derived Protein, TNF Protein, monocyte derived Protein, TNF-a Protein, TNF-alpha Protein, TNFA Protein, TNFA_HUMAN Protein, TNFSF2 Protein, Tumor necrosis factor Protein
- SDS-PAGE
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