Products
Recombinant Mouse LIF
- Product Name
- Recombinant Mouse LIF
- Catalogue No.
- P4609
- Species
- Mouse
- Host
- E.Coli
- Expression Region
- 24-203
- Mol. Weight
- 19.7 kDa
- Tags
- N-terminal His Tag
- Purity
- Greater than 95% as determined by SDS-PAGE
- Application
- Western Blot, ELISA
- Activity
- Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells. The ED50 for this effect is 0.2-0.6 ng/mL.
- Formulation
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4
- Reconstitution
- Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
- Storage
- The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
- Alternative Names
- CDF Protein, Cholinergic Differentiation Factor Protein, D factor Protein, DIA Protein, Differentiation inducing factor Protein, differentiation inhibitory activity Protein, Differentiation stimulating factor Protein, Differentiation-stimulating factor Protein, Emfilermin Protein, Hepatocyte stimulating factor III Protein, HILDA Protein, Human interleukin in DA cells Protein
- SDS-PAGE
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