Recombinant rat PDGFB

Alternative Names:Becaplermin Protein, c sis Protein, FLJ12858 Protein, PDGF 2 Protein, PDGF subunit B Protein, PDGF2 Protein, PDGFB Protein, PDGFbb Protein, Proto oncogene c Sis Protein, SIS Protein, SSV Protein
Catalogue No.:P3244Uniprot ID:Q05028
Host:E.ColiExpression Region:74-182
Mol. Weight:14.1 KDaTags:N-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant rat PDGFB
Catalogue No.
P3244
Species
Rat
Host
E.Coli
Expression Region
74-182
Mol. Weight
14.1 KDa
Tags
N-terminal His Tag
Purity
Greater than 90% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells.The ED50 for this effect is 2-10 ng/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
Becaplermin Protein, c sis Protein, FLJ12858 Protein, PDGF 2 Protein, PDGF subunit B Protein, PDGF2 Protein, PDGFB Protein, PDGFbb Protein, Proto oncogene c Sis Protein, SIS Protein, SSV Protein
SDS-PAGE