Recombinant Human CXCL1

Alternative Names:CINC1 Protein, CINC-1 Protein, CXCL1 Protein, GRO alpha Protein, KC Protein, MGSA-alpha Protein
Catalogue No.:P0207Uniprot ID:P09341
Host:E.ColiExpression Region:35-107
Mol. Weight:9.7 KDaTags:N-terminal His Tag
  • SPECIFICATIONS
Product Name
Recombinant Human CXCL1
Catalogue No.
P0207
Species
Human
Host
E.Coli
Expression Region
35-107
Mol. Weight
9.7 KDa
Tags
N-terminal His Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. The ED50 for this effect is 0.5-2 μg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
CINC1 Protein, CINC-1 Protein, CXCL1 Protein, GRO alpha Protein, KC Protein, MGSA-alpha Protein
SDS-PAGE