Recombinant Mouse TNFA

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Alternative Names:APC1 protein Protein, Cachectin Protein, Cachetin Protein, DIF Protein, TNF Protein, TNF Protein, monocyte-derived Protein, tnfa Protein, tnf-a Protein, TNFalpha Protein, TNF-alpha Protein, TNF-alphacachectin Protein, TNFATNF Protein, macrophage-derived Protein
Catalogue No.:P0157Uniprot ID:P06804
Host:E.ColiExpression Region:80-235
Mol. Weight:17.2 kDaTags:N-terminal His Tag
  • SPECIFICATIONS
  • FIGURES
  • CONDITIONS
Product Name
Recombinant Mouse TNFA
Catalogue No.
P0157
Species
Mouse
Host
E.Coli
Expression Region
80-235
Mol. Weight
17.2 kDa
Tags
N-terminal His Tag
Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
< 1 EU/μg as determined by LAL test.
Application
Western Blot, ELISA
Activity
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin. The ED50 for this effect is 20-60 pg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.
Reconstitution
Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Storage
The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
Alternative Names
APC1 protein Protein, Cachectin Protein, Cachetin Protein, DIF Protein, TNF Protein, TNF Protein, monocyte-derived Protein, tnfa Protein, tnf-a Protein, TNFalpha Protein, TNF-alpha Protein, TNF-alphacachectin Protein, TNFATNF Protein, macrophage-derived Protein
SDS-PAGE