Products
Recombinant Mouse TNFA

Category:
Alternative Names: | APC1 protein Protein, Cachectin Protein, Cachetin Protein, DIF Protein, TNF Protein, TNF Protein, monocyte-derived Protein, tnfa Protein, tnf-a Protein, TNFalpha Protein, TNF-alpha Protein, TNF-alphacachectin Protein, TNFATNF Protein, macrophage-derived Protein | ||
Catalogue No.: | P0157 | Uniprot ID: | P06804 |
Host: | E.Coli | Expression Region: | 80-235 |
Mol. Weight: | 17.2 kDa | Tags: | N-terminal His Tag |
- SPECIFICATIONS
- FIGURES
- CONDITIONS
- Product Name
- Recombinant Mouse TNFA
- Catalogue No.
- P0157
- Species
- Mouse
- Host
- E.Coli
- Expression Region
- 80-235
- Mol. Weight
- 17.2 kDa
- Tags
- N-terminal His Tag
- Purity
- Greater than 90% as determined by SDS-PAGE.
- Endotoxin
- < 1 EU/μg as determined by LAL test.
- Application
- Western Blot, ELISA
- Activity
- Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin. The ED50 for this effect is 20-60 pg/mL.
- Formulation
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.
- Reconstitution
- Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
- Storage
- The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
- Alternative Names
- APC1 protein Protein, Cachectin Protein, Cachetin Protein, DIF Protein, TNF Protein, TNF Protein, monocyte-derived Protein, tnfa Protein, tnf-a Protein, TNFalpha Protein, TNF-alpha Protein, TNF-alphacachectin Protein, TNFATNF Protein, macrophage-derived Protein
- SDS-PAGE
-