- Catalog No.
K138
- Size
20T/50T/100T
- Kit components
|
Reagents
|
20T
|
50T
|
100T
|
|
Annexin V-FineTest®Violet 450
|
100μL
|
250μL
|
500μL
|
|
1X Binding Buffer
|
10mL
|
25mL
|
50mL
|
|
Propidium Iodide (PI)
|
200μL
|
500μL
|
1mL
|
- Storage
2-8°C for 12 months. Annexin V-FineTest®Violet 450 and propidium iodide need to be stored away from light.
- Shipping Conditions
ice pack
- Description
Annexin V-FineTest®Violet 450/PI apoptosis detection kit can be used to detect apoptosis in suspension cells and adherent cells.
Annexin V is a calcium-dependent phosphatidylserine binding protein with a high affinity for phosphatidylserine PS. Annexin V labelled FineTest®Violet 450 can bind to the membrane of early apoptotic cells by means of the PS exposed outside the cells. Apoptosis can be detected by flow cytometry or fluorescence microscopy.
Propidium Iodide (PI) binds specifically to double-stranded DNA and produces strong fluorescence, which is normally unable to penetrate cell membranes. Due to late apoptotic or necrotic cell membrane loss of integrity, PI can enter cells to stain DNA and, when used in combination with Annexin V, distinguish cells at different stages of apoptosis.
The following figure shows the apoptosis effect of Jurkat cells induced by camptothecin detected by this kit.
- Note
1.It should be detected as soon as possible after staining. Too long a time may lead to an increase in the number of apoptotic or necrotic cells.
2.When detecting adherent cells, the suspension cells generated after inducing apoptosis should be collected and detected together with the adherent cells collected later.
3.Mechanical damage caused by digestive adherent cells should be avoided as much as possible. At the same time, the digestive juices of pancreatic enzymes should be as free of EDTA as possible, as EDTA affects Annexin V binding to phosphatidylserine.
4.If EDTA-containing pancreatic enzymes are used, the cells should be thoroughly washed after collection to ensure that EDTA is removed.
5.Fluorescent substances are prone to quenching, in the fluorescence observation, as far as possible to shorten the observation time, while in the operation and storage process also try to pay attention to the preservation of light.
6.For your safety and health, please wear a lab coat and disposable gloves.
7.This kit is for scientific research only.
