- Catalog No.
K136
- Size
2mL(0.5mL settled Beads)
- Storage
2-8°C for 12 months. Do not freeze.
- Description
Streptavidin MagBeads are highly suitable for the purification of biotinylated proteins, peptides, antibodies, and oligonucleotides, as well as for protein interaction studies.
Streptavidin and biotin have an exceptionally high affinity (Kd = 10⁻¹⁵), which underpins their widespread use in biological applications. By adding Streptavidin MagBeads to a sample containing biotinylated molecules, the target binds to the beads after a brief incubation. The complex can then be separated using a magnetic stand for purification purposes.
These Streptavidin MagBeads are superparamagnetic beads approximately 45–100 μm in size, covalently coated with high-purity streptavidin. Streptavidin is a 53 kDa protein with four biotin-binding sites per molecule. It is stable over a pH range of 2–14 for short-term exposure and 3–13 for working conditions. The beads are supplied as a 25% slurry in phosphate-buffered saline (PBS), pH 7.4, containing 20% ethanol.
- Recommended Buffers and Solutions
|
For Nucleic Acid Coupling
|
For Protein or Antibody Coupling
|
|
TES Buffer:
10 mM Tris
1 mM EDTA
2 M NaCl
pH 7.5
|
PBS Buffer pH 7.4
|
- Binding Capacity
1 mL of pure Streptavidin MagBeads has a binding capacity of: Free Biotin > 60 nmol.
- Materials to Be Prepared by the User
Vortex mixer or rotator mixer
Magnetic separation stand
Pipettes and tips, suitable centrifuge tubes
- Note
This product is for research use only. Unless otherwise specified, it is not intended for any animal or human therapeutic or diagnostic use. This product contains 20% EtOH as a preservative.
