Products
Influenza A virus (H5N1) NS1 protein Antibody
| Synonyms： | Influenza A virus (H5N1) NS1 protein|Non-structural protein 1|NS1|NS1A|NS|influenza A virus (H5N1) Non-structural protein 1| antibody | ||
| Catalogue No.： | FNab11150 | Reactivity： | Influenza A virus (H5N1), Influenza A virus (H1N1) |
| Host： | Mouse | Tested Application： | ELISA,WB |
| Clonality： | Monoclonal | Isotype： | IgG1 |
| Size | Price |
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- SPECIFICATIONS
- FIGURES
- CONDITIONS
- FAQS
- Product Name
- Influenza A virus (H5N1) NS1 protein Antibody
- Catalogue No.
- FNab11150
- Size
- 100µg
- Form
- liquid
- Purification
- Protein A/G purified from cell culture supernatant
- Purity
- ≥90% as determined by SDS-PAGE
- Clonality
- Monoclonal
- Isotype
- IgG1
- Clone ID
- 2H6
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)
- Immunogen
- Influenza A virus (H5N1) NS1 protein
- Alternative Names
- Influenza A virus (H5N1) NS1 protein|Non-structural protein 1|NS1|NS1A|NS|influenza A virus (H5N1) Non-structural protein 1| antibody
- UniProt ID
- O56264
- Observed MW
- 26 kDa
- Tested Applications
- ELISA,WB
- Recommended dilution
- ELISA: 1:2000-1:20000;WB: 1:500-1:2000
- Background
- Non-structural protein 1 (NS1) is a ~26 kDa protein. Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor/CPSF4 and the poly(A)-binding protein 2/PABPN1. In turn, unprocessed 3' end pre-mRNAs accumulate in the host nucleus and are no longer exported to the cytoplasm. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism. Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated RIGI ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors IRF3 and IRF7.
How many times can antibodies be recycled?
First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.
Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.
What are components of FineTest antibody buffer?
Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.
How about the storage temperature and duration of FineTest antibodies?
Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.
Is dilution required for FineTest antibodies? What’s the dilute solution?
Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).
How to retrieve antibodies for immunohistochemistry?
Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)
Heat induced antibody retrieval:
Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;
Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.
How to choose secondary antibodies?
(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.
(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).