Products

GPX2 antibody

Synonyms:GPX2 antibody, GI-GPx antibody, GPRP antibody, GPRP-2 antibody
Catalogue No.:FNab10491Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
GPX2 antibody
Catalogue No.
FNab10491
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
Glutathione peroxidase 2
Alternative Names
GPX2 antibody, GI-GPx antibody, GPRP antibody, GPRP-2 antibody
UniProt ID
P18283
Observed MW
21 kDa
Application
Tested Applications
ELISA, WB, IHC
Recommended dilution
WB: 1:500-1:2000; IHC: 1:50-1:200
Validated Images
Mouse stomach tissue were subjected to SDS PAGE followed by western blot with FNab10491(GPX2 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon carcinoma slide using FNab10491(GPX2 antibody) at dilution of 1:50
Background
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract (also in liver in human), is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Overexpression of this gene is associated with increased differentiation and proliferation in colorectal cancer. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.