Products

Amphiregulin antibody

Synonyms:Amphiregulin antibody, AR antibody, AREG antibody, AREGB antibody, CRDGF antibody, SDGF antibody
Catalogue No.:FNab10384Reativity:Human, rat
Host:RabbitTested Application:ELISA, WB, IHC
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
Amphiregulin antibody
Catalogue No.
FNab10384
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
amphiregulin
Alternative Names
Amphiregulin antibody, AR antibody, AREG antibody, AREGB antibody, CRDGF antibody, SDGF antibody
UniProt ID
P15514
Observed MW
43-50 kDa
Application
Tested Applications
ELISA, WB, IHC
Recommended dilution
WB: 1:200-1:2000; IHC: 1:50-1:500
Validated Images
MCF-7 cells were subjected to SDS PAGE followed by western blot with FNab10384(AREG Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded rat lung slide using FNab10384(AREG Antibody) at dilution of 1:100
Background
Amphiregulin(AREG) is one of the ligands of the epidermal growth factor receptor(EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms(PMID: 9642297).