Products
ECD antibody
- Product Name
- ECD antibody
- Catalogue No.
- FNab10368
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- ecdysoneless homolog(Drosophila)
- Alternative Names
- ECD antibody, GCR2 antibody, HSGT1 antibody, Protein ecdysoneless homolog antibody, Protein SGT1 antibody, SGT1 antibody, Suppressor of GCR2 antibody
- UniProt ID
- O95905
- Observed MW
- 70-100 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab10368(ECD antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon tissue using FNab10368(ECD antibody) at dilution of 1:100
- Background
- Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP(PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes(PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins(PubMed:19640839). The cell cycle-regulating function may depend on its RUVBL1-mediated association with the R2TP complex(PubMed:26711270). May play a role in regulation of pre-mRNA splicing(PubMed:24722212).
