Products

anti- FOXO1 antibody

FNab10255 Catalogue No.
Synonyms
FKHR, FOXO1A
Host
Rabbit
Reactivity
Human
Isotype
IgG
Tested Application
ELISA, WB
Product Information
Product Name
anti- FOXO1 antibody
Catalogue No.
FNab10255
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element(IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element(DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner.
Immunogen Information
Immunogen
forkhead box O1
Synonyms
FKHR, FOXO1A
Observed MW
70-80 kDa
Application
Tested Application
ELISA, WB
Recommended dilution
WB: 1:200-1:2000
Uniprot information
UniProt ID
Q12778
Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab10255(FOXO1 Antibody) at dilution of 1:600