Products

anti- SHARPIN antibody

FNab10251 Catalogue No.
Synonyms
hSIPL1, SHARPIN, SIPL1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Tested Application
ELISA, WB, IHC
Product Information
Product Name
anti- SHARPIN antibody
Catalogue No.
FNab10251
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex(TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
Immunogen Information
Immunogen
SHANK-associated RH domain interactor
Synonyms
hSIPL1, SHARPIN, SIPL1
Observed MW
40 kDa
Application
Tested Application
ELISA, WB, IHC
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
Uniprot information
UniProt ID
Q9H0F6
Images
Immunohistochemistry of paraffin-embedded rat kidney tissue slide using FNab10251(SHARPIN Antibody) at dilution of 1:100
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab07838(SHARPIN antibody) at dilution of 1:500