Products

anti- TNFAIP3 antibody

FNab10156 Catalogue No.
Synonyms
A20, OTUD7C, TNF alpha induced protein 3, TNFA1P2, TNFAIP3, Zinc finger protein A20
Host
Rabbit
Reactivity
Human, rat
Isotype
IgG
Tested Application
ELISA, WB, IHC
Product Information
Product Name
anti- TNFAIP3 antibody
Catalogue No.
FNab10156
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors(TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF-or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor(TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM(CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase(IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'-and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.
Immunogen Information
Immunogen
tumor necrosis factor, alpha-induced protein 3
Synonyms
A20, OTUD7C, TNF alpha induced protein 3, TNFA1P2, TNFAIP3, Zinc finger protein A20
Observed MW
90 kDa, 40 kDa
Application
Tested Application
ELISA, WB, IHC
Recommended dilution
WB: 1:500-1:2000; IHC: 1:50-1:500
Uniprot information
UniProt ID
P21580
Images
Immunohistochemistry of paraffin-embedded rat kidney using FNab10029(TNFAIP3 antibody) at dilution of 1:100
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab10156(TNFAIP3 antibody) at dilution of 1:1000