Products

anti- c-Kit/CD117 antibody

FNab09971 Catalogue No.
Synonyms
C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Tested Application
ELISA, WB, IHC
Product Information
Product Name
anti- c-Kit/CD117 antibody
Catalogue No.
FNab09971
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK(isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
Immunogen Information
Immunogen
Mast/stem cell growth factor receptor Kit
Synonyms
C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit
Observed MW
120 kDa, 145 kDa
Application
Tested Application
ELISA, WB, IHC
Recommended dilution
WB: 1:200-1:2000; IHC: 1:50-1:200
Uniprot information
UniProt ID
P10721
Images
Immunohistochemistry of paraffin-embedded rat ovary tissue slide using FNab09971(C-KIT Antibody) at dilution of 1:100
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab09971(c-Kit/CD117 antibody) at dilution of 1:200