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AMOT antibody
- Product Name
- AMOT antibody
- Catalogue No.
- FNab09852
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- angiomotin
- Alternative Names
- AMOT antibody, angiomotin antibody, KIAA1071 antibody
- UniProt ID
- Q4VCS5
- Observed MW
- 80 kDa, 130 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:50-1:500; IF: 1:20-1:200
Validated Images
HEK293 cells were subjected to SDS PAGE followed by western blot with FNab09852(AMOT Antibody) at dilution of 1:1000
IP Result of anti-AMOT (IP:FNab09852, 4ug; Detection:FNab09852 1:300) with HEK-293 cells lysate 2500ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab09852(AMOT Antibody) at dilution of 1:200
Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HEK-293 cells using FNab09852(AMOT Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Angiomotin belongs to the motin family of angiostatin binding protein. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Most abundant expression was found in placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). The p130 isoform can interact with F-actin.This antibody recognizes both p130 and p80.
